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ICD is one of the most widely studied methods of immunotherapy, which induces and activates the adaptive immune response mediated by T cells through the production of tumor-associated antigens (TAAs) and the release of damage-associated molecular patterns (DAMPs) [143, 144] Recent studies have shown that CDT-induced oxidative stress can induce ICD effects in tumors [145, 146]

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Additionally, these cells showed upregulation of the synthesis of ser/gly synthesis-derived nucleotides, as indicated by the enrichment of m + 6 isotopologues, i.e., AMP in 1919 PSPH amp cells, CTP in 1919 PSPH V116I cells and GTP in both cell models, compared to 1919 control cells (Fig