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The beads were washed 4 times with ice-cold RIPA buffer (50 mM HEPES, pH = 7.5, 500 mM LiCl, 1 mM EDTA, 1.0% NP-40, 0.7% Na-Deoxycholate), followed by once with ice-cold TE buffer (with 50 mM NaCl)

4.8 Amino acid-associated post-translational modifications Post-translational modifications (PTMs) of proteins, occurring primarily on specific amino acid residues such as serine, threonine, tyrosine, lysine, and cysteine, play a crucial role in expanding the functional diversity of the plant proteome by modulating protein activity, stability, localization, and interactions

Count carefully from the nearest labeled number

Our objectives were to: Provide superior intracellular absorption Create a heat-stable product that does not require refrigeration