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In the catabolic route, 5-FU is converted into dihydro fluorouracil (DHFU) by dihydropyrimidine dehydrogenase (DPD) (mostly in the liver), forming -fluoro-- alanine (FBAL) and -fluoro--ureido propionic acid (FUPA) which are excreted through kidneys (Peters, 2020)

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Though this method is not typically used to measure cytokine levels as compared to the more common plasma measurements, it can be an important method of measure due to the short half-life of cytokines and their localization in production, thus many studies have utilized this alternate method (Sullivan et al., 2000