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Approximately 100 ng of the DNA fragment was incubated with the purified protein in a reaction mixture containing 1X binding buffer (15 mM HEPES, pH 7.5, 35 mM KCl, 1 mM MgCl2, 1 mM EDTA, pH 8.0, 2% glycerol, and 1 mM DTT) in a total volume of 20 l