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An alternative approach that has been explored experimentally in the porcine ischemic injury model to limit formation of LA inhibitory fragments while maintaining the ability of LA to induce recovery of barrier function involves addition of small doses of LA over time and suggest that 0.1 M LA applied every 45 min is effective (49)

however, in damaged mitochondrial fragments, PINK1 cleavage is inhibited, and its 63 kDa isoform accumulates on the OMM where it phosphorylates cytoplasmic Parkin and ubiquitin resulting in the recruitment of the mitophagic machinery to carry out degradation 32

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Bafilomycin or DMSO was added to the wound healing assay 30 min before EV addition