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Actually, BAT is an oxidative tissue that, when fully activated, uses fatty acids stored into brown adipocytes to support the activity of the uncoupling protein 1 (Ucp1 or thermogenin) that dissipates the mitochondrial proton gradient to produce heat

The RAW 264.7 cells were treated with FONE (0, 16.7, and 20 g/mL), with catalase (0, 5, and 15 U/mL) or 3-AT (0, 5, and 15 U/mL) for 24 h and then subjected for the Cyto-ID autophagy detection

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