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Collagen gel contraction assays Fibroblast cultures at passage 3 were cultured in six-well tissue culture plates (Gibco, Canada) in growth medium (DMEM, 10% FBS, penicillin, and streptavidin from Invitrogen, Canada)

SAM S-adenosyl-L-[methyl-3H] methionine PRMT3 activity assay A radiometric assay was performed by RBC using 100 M-5 nM SAH as an inhibitor positive control

Special thanks to Dr Louis-Charles Fortier for critical reading of the manuscript and preparation of material for this review and Dr Claude Labrie for critical reading

Delivery intervals are diverse, too, ranging from as little as two weeks to as long as six months