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Description

Sural, S., Liang, C

Candidate clones underwent three rounds of subcloning to establish stable lines (passages T1-T3), which were then expanded from 96-well plates to 6-well plates and T25 flasks

For this purpose, we use several biophysical methods, including cryo-electron microscopy (cryo-EM), small-angle X-ray scattering (SAXS), hydrogen/deuterium exchange coupled to mass spectrometry (HDX-MS), sedimentation velocity analytical ultracentrifugation (SV-AUC), and functional assays