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Primary antibodies were then added and incubated at 4C overnight

TMED4 is a potent activator of NRF2, and loss of TMED4 prevents NRF2 from inducing an antioxidant state within the cell

The mustard seed extract was testified for APAP-induced toxicity in a hepatocellular carcinoma cell line (HepG2) and was found to have protective effects in both post and pretreatment models with a 20 mM APAP dose (Parikh et al., 2015)