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Moreover, hypothermia suppresses or delays the production of important innate immune components in adult fish in response to pathogen mimics or pathogens, blocking proinflammatory or antiviral responses (Abram et al

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GGT was analysed by adding 2ml buffer (Tris HCl 120 mm , MgCl2 12mM glycyl glycine 90 mM , pH 7.8) to 0.2ml substrate (L- g-glutamyl p-nitro anilide 48 mm in 150mM HCl ), warmed to 37C