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2e), implying that the cnd1-K658E mutation is sufficient to cause chromosome segregation defects without affecting cell viability at a detectable level

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Electron microscopy sample preparation and data acquisition A 3Ll volume of 5LmgLml 1 purified SLC33A1 MAP was mixed with Pmab1 Fv-clasp at a 1:1.5 molar ratio and 20mM GSSG, incubated for 30 min on ice and then applied to glow-discharged Au 400 mesh QUANTIFOIL R1.2/1.3 holey carbon grids (Quantifoil) or Graphene Oxide Au 400 mesh QUANTIFOIL R1.2/1.3 grids before being plunged into liquid-nitrogen-cooled liquid ethane using an FEI Vitrobot Mark IV (FEI Thermo Fisher)

Cytokine production after LPS induction was measured with ELISA kits for IL-6 (R&D, M6000B-1) and TNF (BioLegend, 430907)