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Cell pellets were then lysed in 50 l of 5% SDS, 100 mM TEAB and sonicated (QSonica) for 5 cycles, with a 1 min resting period on ice after each cycle

Carrier-mediated transport involves the binding of the target molecules to the receptor portion of the transporters, which in turn produces a conformational change in the carrier and allows the translocation of the compound (147)

Patients in the study group received education in symptom control and natural history of ESLD using visual aids

RNA extraction and RNAsequence analysis RNA was extracted from snap-frozen cancer tissues from 39 ovarian cancer specimens using the AllPrep DNA/RNA Mini Kit according to the manufacturers instructions (Qiagen, Hilden, Germany) and 500 ng were used for sequencing