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The E167K variant enhances the interaction between TM6SF2 and the PNPLA3 variant I148M, which inhibits the function of PNPLA3 in mediating the transfer of polyunsaturated fatty acids (PUFAs) from TG to phosphatidylcholine, leading to increased accumulation of polyunsaturated TG in the liver and exacerbation of liver injury [271]

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LC-MS-based metabolite detection For determination of the relative Cys and GSH pool sizes across compartments, previously established LC-MS conditions were employed 5,24 For chromatographic metabolite separation, a Vanquish UPLC system was coupled to a Q Exactive HF (QE-HF) mass spectrometer equipped with HESI (Thermo Fisher Scientific, Waltham, MA, USA)