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Persister Cell Derivation and Treatment Persister cells were derived from colorectal cancer cell lines HCT116 (KRAS G13D ) and SW620 (KRAS G12V ) treated with 5-FU and AZ628, for at least 9 days with fresh drug added every 3 days, and then stained with crystal violet or counted using a Countess Automated Cell Counter (Invitrogen, Carlsbad, CA, USA)