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Preladenate, however, did not make it to the end of phase 3 clinical trials in the treatment of PD because the compound did not outperform placebo in reducing the off state, and tozadenate was excluded from the phase 3 clinical trial in the year 2018 because of serious side effects, hematologic toxicity such as agranulocytosis (Hauser et al., 2014, 2015

All cell lines were cultured in suspension using RPMI-1640 medium (Gibco) supplemented 10% FBS (New Zerum), under the condition of 37 C and 5% CO 2

In: Molecular Base of Malignancy

All remaining steps were performed using PBS/T/S pH 6.0 or pH 7.4 as dilution and wash buffers