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Interestingly, despite DRP1-induced mitochondrial fission, we did not observe a significant increase in ROS levels, likely due to the activation of the NRF2/SLC7A11/GSH axis, which facilitates ROS clearance

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After SCAPs were treated with CCK8 at 37 C for 2 hours, the optical density was measured by microplate reader scanning at 490 nm according to the manufacturers instruction 52