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Presence of 5 alpha-reductase isozymes and aromatase in human prostate cancer cells and in benign prostate hyperplastic tissue

c-Myc is a transcription factor involved in diverse human malignant tumors 129,130

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To quantify the mScarlet fluorescence in individual chromatography fractions, 100 L samples were each transferred to a 96-well Nunc black microwell plate (Thermo Fisher Scientific) and the fluorescence intensities were measured in a Synergy 2 microtiter plate reader with 530/25 nm and 590/35 nm filters for excitation and emission, respectively