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The microglial cell shifts to the M1 phase during cerebral I/R, due to the release of inflammatory mediators, ROS, DAMPs, and neuromodulators from degenerated neurons in the ischemic core and activation of nuclear factor kappa- (Fernandez-Lizarbe et al

Normally the formation and decomposition of active forms of oxygen are balanced up and do not accumulate in cells

This rapid increase in absorbance is assumed to be due to the leakage of low molecular weight compounds absorbing at 260 nm (ATP, nucleosides, and nucleotides) and at 280 nm (amino acids and small peptides that are free in the cytoplasm)