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Mice lacking radixin, which links actin filaments to plasma-membrane proteins, develop conjugated hyperbilirubinemia associated to retrieval of Mrp2,122 and the same hols true for obstructive and estrogen-induced cholestasis, where a disturbed colocalization of Mrp2 and radixin is associated with Mrp2 endocytic internalization.123 Interestingly, the internalization of Mrp2 that occurs after hepatic ischemia-reperfusion is coincident with a virtual loss of radixin expression.124 Mrp2 retention in the apical membrane also requires interaction with the PDZ-domain protein, PDZK1.125 Since other canalicular transporters, such as Bsep, Mdr1 and Mdr2, do not contain obvious PDZ-interacting motifs, regulation by actin of the localization of these transporters must occur through a different subset of proteins

These cells were subsequently visualized into 12 distinct cell clusters, including macrophage, neutrophil, mast cell, B cell, CD 4+ T cell, CD 8+ T cell, plasma cell, fibroblast, endothelial cell, mesenchymal stem cell (MSC), Schwann cell (ScC), and nonmyelinating ScC (nmScC), by generating uniform manifold approximation and projection (UMAP) plots based on hierarchical clustering and established lineage-specific marker genes (Fig

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