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Since the inhibition of ASK1 (apoptosis signal-regulation kinase 1) in NLRP3 (NOD-like receptor thermal protein domain associated protein 3) mutant mice can reduce liver fibrosis, hepatocyte death, and liver TNF- expression, it suggests the application prospects of ASK1 inhibitor selonsertib in liver diseases

The membranes were incubated with 5% BSA at room temperature for 1 h, then with primary antibodies at 4 C overnight and second antibody successively for 1 h at room temperature, and consequently developed on the imaging system after adding ECL reagent

PMID: 18824438

The spray voltage was set at 3.0 kV and the source temperature was set at 120 C