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H 2 O 2 sensing through oxidation of the Yap1 transcription factor

Isolated epidermal and dermal cells were mixed in a 1:9 ratio and dropped into the upper chamber of transwell cultures, and the lower chamber was filled with 700 l of Dulbeccos modified eagle medium/F12 (#MT100 13CV, Corning, USA) medium (#1099-141C, Gibco, USA) containing 10% fetal bovine serum

This same extract has been shown to enhance adipogenesis of 3T3-L1 cells, a widely used model to study adipocyte differentiation, as measured by both lipid accumulation and adipogenic gene expression ( Given that obesity drives insulin resistance and diabetes, interventions to reduce fat mass have been pursued as a means to counter obesity-associated metabolic disease