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Description

Lactate measurements Lactate was measured in cell media using the Lactate-Glo Assay (Promega)

Single base pair changes or sequence randomization in the linker region between hairpins may result in complete loss of CRISPR complex activity

2002;23 Suppl 3:11-144

A candidate for a potential glutathione-scaffold residue was identified using the following selection criteria: The residue should be rather conserved in enzymatically active in contrast to inactive glutaredoxins and have a charged side chain to ensure a strong ionic glutathione interaction (Fig