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Description

To construct the sorting vector for gene overexpression, we amplified the whole expression cassette of N-signal-GST-EGFP-GPI DAF and N-signal-GST-EGFP-GPI CEAM7 from pEGFP-GST-GPI DAF and pEGFP-GST-GPI CEAM7 vectors and joined them with PCR linearized pcDNA3.1 vector using ClonExpress II One Step Cloning Kit

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Further Reading If you found this post on Gary Breckas peptides helpful, you may also like: Bryan Johnsons Peptide List The peptides the longevity-obsessed entrepreneur has tested, tracked, and in some cases dropped, with his data-driven reasoning for each