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cDNA synthesis of the LASV GPC was performed with the SuperScript IV first strand synthesis system (Thermofisher), followed by PCR using Phusion polymerase (New England Biolabs) and primers 5-GATCCTAGGCTTTTTGGATTGCG-3 and 5-AAGAAAGAGATCACCCCGCACTGT-3

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Common cycling patterns include standard cycles of 8 weeks on followed by 4 weeks off, extended cycles of 12 weeks on followed by 6 weeks off for more aggressive protocols, and conservative cycles of 6 weeks on followed by 3 weeks off for those preferring a cautious approach