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Nave (non-infected) erythrocytes and Plasmodium falciparum 3D7-infected erythrocytes were cultured at 37C, in low oxygen conditions (1% O 2 , 5% CO 2 , 94% N 2 ), at 4% or 0.4% hematocrit, in incomplete RPMI media (iRPMI: RPMI 1640 HEPES, 50 mg/L hypoxanthine, 10 mg/L gentamicin, pH 6.74), complete RPMI media (cRPMI: incomplete RPMI supplemented with 2.25 g/L sodium bicarbonate and 5 g/L AlbuMAX II), or Ringer solution (1mM CaCl 2 , 5mM glucose, 32 mM HEPES pH 7.4, 5 mM KCl, 1mM MgSO4, 125mM NaCl)

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Many users are able to build up to nightly use as tolerated.