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223 Wnt signalling activated by RNF43 mutation promotes tumour growth and a high recurrence rate in CRC patients

While classical antifolates like methotrexate inhibit DHFR and nucleotide synthesis [164], recent drug development has expanded to other key enzymes such as MTHFD2, PHGDH, and SHMT2, with inhibitors now in clinical or preclinical development, as summarized in Table 1