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Because the amount of spine elimination is more than two-fold higher than that of spine formation between adolescence and adulthood, these in vivo studies indicate that the net spine loss observed in fixed tissue is mainly due to the elimination, also called pruning, of existing synaptic connections

1b ) which develop into type 2 cells and type C cells, respectively and the sequence and expression profile of TFs essential for NSC fate determination in adult neurogenesis [60, 61]

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Reported side effects are minimalprimarily mild nasal irritation with intranasal administration