Immunoblot assays Tissue samples were crushed on dry ice to obtain homogenous aliquots and then lysed in RIPA buffer (Thermo Scientific #89900) containing Halt protease & phosphatase inhibitor (Thermo Scientific #1861280)
Other plant defense chemicals capable of triggering Leaky Gut Syndrome include lectins and oxalates, which are found in many vegetables, grains and legumes
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Disclosure of interest: The authors of this study have nothing to disclose Poster Session I - RISK FACTORS - PRIMARY AND SECONDAR PREVENTION 05.00 - RISK FACTORS AND PREVENTION (PRIMARY AND SECONDARY) - 05.04 - STROKE COMPLICATIONS P403 - ESOC25-2347 POST-STROKE EPILEPSY IN THE ERA OF ADVANCED REPERFUSION THERAPIES Catarina Guedes Vaz 1 , Alexandre Dias 2,3 , Ricardo Varela 1 , Isabel Silva 3 , Ana Moutinho 2,3 , Pedro Castro 4 , Bruno Castro 4 , Carla Oliveira 2,5 , Henrik Zetterberg 6 , Johan Gobom 6 , Manuel Correia 1,3 , Rui Magalhes 3 , Lus Maia 1,2,3 1 Servio de Neurologia, Centro Hospitalar Universitrio de Santo Antnio, ULSdSA, Porto, Portugal, 2 Instituto de Investigao e Inovao em Sade (i3S), Universidade do Porto, Porto, Portugal, 3 Instituto de Cincias Biomdicas Abel Salazar (ICBAS), Universidade do Porto, Porto, Portugal, 4 Servio de Neurologia, Centro Hospitalar Universitrio de So Joo, ULSdSJ, Porto, Portugal, 5 Institute of Molecular Pathology and Immunology of the University of Porto (IPATIMUP), Porto, Portugal, 6 Institute of Neuroscience and Physiology, Department of Psychiatry and Neurochemistry, University of Gothenburg (UGOT), Gothenburg, Sweden Background and Aims: Post-stroke epilepsy (PSE) is a significant comorbidity in stroke survivors
