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Assessment of Mitochondria Membrane Potential (MMP) Acute CO slices were incubated with JC-1 ((5,5,6,6-tetrachloro-1, 1,3,3-tetraethylbenzimi- dazolylcarbocyanine iodide) Dye (Invitrogen, Mitochondrial Membrane Potential Probe, T3168) at a concentration of 1 g/mL for 30 min in the dark on an orbital shaker at 37 C with 5% CO 2 and humidity