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Over the past few decades, various diagnostic frameworks have been established, with the most widely adopted being the 1990 National Institutes of Health (NIH) criteria, the 2003 Rotterdam Consensus, and the 2006 Androgen Excess and PCOS Society (AES) criteria

For instance, synthetic drugs are often derived from chemical reagents or scarce natural products dependent on non-renewable resources, whereas live bacterial drugs are predominantly sourced from readily available, biodegradable natural material [14, 15]

Previous studies have shown that inhibiting SIRT2 may enhance the stability of glucokinase (GCK) regulatory proteins, accelerate aldolase A degradation, and inhibit -cell insulin secretion via modulation of the Akt/glycogen synthase kinase-3 (GSK3)/-catenin pathway, collectively reducing glycolytic flux (42)

The change in biochemical indicators and their trend is important in assessing the stress condition in birds and to implement the effective mitigation strategies to combat stress