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Dysbiotic disruptions occur as consequences of critical illnesses such as cancer, cardiovascular and chronic kidney disease but also neurological, mental, and pain disorders, as well as ischemic and traumatic brain injury

VSV-G-possessing EVs in s-EV fractions exhibited enhanced delivery efficiency 13, 15, 16 , and the protrusion-derived EVs without VSV-G in this study were considered to achieve similar but still weaker delivery efficiency to the EVs carrying VSV-G (Supplementary Table 1)

Therefore, we performed Western blot analysis to measure the protein levels of the biomarkers of apoptosis and DNA damage such cleaved poly (ADP-ribose) polymerase-1 (PARP-1) and cleaved caspase-3