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We consistently obtained 9098% of SRY-box Transcription Factor 17 (SOX17) + and FOXA2 + definitive endoderm (DE) cells at day 4, 75% of cells expressing a posterior foregut (PFG) marker Hematopoietically Expressed Homeobox (HHEX) at day 6, 8090% pancreatic and duodenal homeobox 1 (PDX1) + and SRY-Box Transcription Factor 9 (SOX9) + pancreatic endoderm (PEN), 6880% pancreatic progenitors (PP) co-expressing PDX1 and NK6 Homeobox 1 (NKX6-1)-at day 12, 65% of Chromogranin-A (CHGA) + and PDX1 + endocrine progenitors (EP) and 3050% cells co-expressing C-peptide (C-PEP) and NKX6-1 (Fig