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Trastuzumab treatment improves brain metastasis outcomes through control and durable prolongation of systemic extracranial disease in HER2-overexpressing breast cancer patients

Juvenile and adult worms were carefully washed in phenol red free RPMI medium and subsequently incubated in phenol red free RPMI medium supplemented with 5.5 mM D-glucose, penicillin-streptomycin and 5% heat inactivated fetal bovine serum and at 37 C in a 5% CO 2 incubator overnight

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Classification into nonpolar/hydrophobic (aliphatic and aromatic), polar uncharged, acidic, and basic categories provides a working framework, though quantitative hydropathy scales such as Kyte-Doolittle and Hopp-Woods more rigorously capture side chain polarity by measuring free energies of transfer between aqueous and nonpolar environments