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After washing with PBS, the cells were treated with Alexa Fluor 488-conjugated secondary antibodies (Invitrogen) for 1 h and subsequently stained with 4,6-diamidino-2-phenylindole (DAPI, Invitrogen) at room temperature

See the classic discussion of UALs role in gynecomastia planning by Rohrich and colleagues: Defining the role of ultrasound-assisted liposuction (PubMed)

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Further experiments showed that colchicine treatment reduced vascular inflammation also in post-myocardial infarction accelerated atherosclerosis through similar mechanisms as documented in early atherosclerosis