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2), the peak fitting algorithm (without apodization) of the ACD/NMR Processor Academic Edition v12.01 software package (Advanced Chemistry Development, Toronto, ON, Canada) was used

The supernatant was collected and injected into the UHPLC-MS/MS system (Thermo Fisher) with a Hypesil Gold column (C18, 100 2.1 mm, and 1.9 m) for analysis