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In vivo kinase assay 100 g of pUGW-CPK5-HA or pUGW-ILK5-HA(WT or S192A) constructs were introduced by polyethylene glycol (PEG)-mediated transformation into Arabidopsis protoplasts prepared from leaf tissues of three- to four-week-old plants described above, then incubated in a 21C growth chamber for 48 h under dark conditions with 2 mL W5 solution (2 mM MES pH 5.7, 154 mM NaCl, 125 mM CaCl 2 , and 5 mM KCl)

These changes may be driven by mutations in oncogenes and tumor suppressor genes, which reprogram cellular metabolism to systematically meet the multifaceted biological demands of tumor cells, such as rapid proliferation, apoptosis resistance, and adaptation to microenvironmental stress including hypoxia and nutrient deprivation [8, 9]

Molecular Weight: 663.43 DA