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GAPDH Glyceraldehyde-3-phosphate dehydrogenase Sense: TGGCAAGTTCAACGGCACAGT Antisense: TTTGGCCTCACCCTTCAGGT 193 bp XM_221353 iNOS (NOS-2) Inducible nitric oxide synthase Sense: TTGGAGCGAGTTGTGGATTGTTGTTC Antisense: GGTGAGGGCTTGCCTGAGTGAGC 126 bp NM_012611 eNOS (NOS-3) Endothelial nitric oxide synthase Sense: CTGGCAAGACCGATTACACGA Antisense: TCAGGAGGTCTTGCACATAGG 206 bp NM_021838 COX-2 Cyclooxygenase-2 Sense: CTGTATCCCGCCCTGCTGGTG Antisense: CCACTTCTCCTCCGAAGGTGC 157 bp AF233596 Biomedicines 2022,10, 265 8 of 38 2.12

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Oral NAD+ Supplements: Because NAD+ itself isnt easily absorbed if taken as a pill, oral NAD+ therapy usually means taking NAD precursors such as nicotinamide riboside (NR) or nicotinamide mononucleotide (NMN)