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Subsequently, the ATP6AP1L coding sequence was amplified from the cDNA of T47D cells and inserted into the FastDigest KpnI/FastDigest XbaI (Thermo Fisher) linearized pcDNA3.1-GST DAF and pcDNA3.1-GST CEAM7 vectors using T4 DNA ligase (EL0011, Thermo Fisher)

At all three conditions during the time analysis period, decreased peak area of NAC parent peak and appearance of additional Di-NAC peak due to possible oxidation product were observed

Overall, these data identify a novel strategy used by influenza virus to induce oxidative stress and to favor its replication in host cells

These agents may be administered from separate solutions if administration of these agents is desirable