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BSO treatment was performed similarly as for ITCs by adding it (100 mM stock solution prepared in water) to the medium (either on its own or in combination with ITC) to obtain BSO concentrations ranging from 50 M to 700 M

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the Jumonji C domain-containing histone demethylase, which catalyzes the demethylation of mono-, bi-, and trimethyl lysine residues by oxidation