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4 Discussion 4.1 Regulatory effects of kaempferol on lipid metabolism in late-laying hens The liver is a vital metabolic organ in laying hens, involved in a variety of key physiological processes including fatty acid synthesis, bile secretion, endogenous lipid anabolism and catabolism, yolk precursor production, and detoxification of metabolic wastes ( ANGPTL3 ), thereby relieving its inhibitory effect on lipoprotein lipase (LPL)

For the acute cold tolerance test, food was removed at the time of transfer and body temperature was measured at the indicated time points

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