glutathione assay calculation Total (GSSG/GSH) Kit Glutathione Quantification GSSG/GSH Quantification Kit

An a priori sample size calculation was performed using G*Power software (version 3.1, University of Kiel, Germany), based on an anticipated large effect size (Cohens d = 0.80), = 0.05, and 80% power, resulting in a minimum of 48 participants per group

10.1016/j.xgen.2025.100768 Cell Genom

and Siren, A-L

Breaks make sense for users experiencing treatment plateau where benefits cease increasing despite continued application suggesting diminishing returns from ongoing use, comfortable with some benefit decline during off periods accepting fluctuation in skin quality, budget-conscious seeking to minimize annual treatment costs through intermittent purchasing, philosophically preferring avoiding indefinite dependency on any skincare product, planning life changes incompatible with consistent application (extended travel, relocation, financial shifts), and wanting to assess whether improvements prove durable or require continuous maintenance before committing to indefinite treatment