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It is very important that ghrelin has to be acylated by ghrelin O-acyl-transferase (GOAT), which binds a fatty acid side chain (C8) to serine 3 of ghrelin, forming acyl ghrelin, the active form that acts on its receptor, a G-protein-coupled receptor (GHSR-1a)

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Meanwhile, 1 mg/L of MT maximized astaxanthin content, reaching 0.3 mg/g DCW and 93.9% higher than the MT-free condition