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There were two papers with low detection bias, while another five articles were rated as unclear with regard to this bias

In the process of sperm selection (e.g., density gradient centrifugation), PDEVs can protect the integrity of sperm membranes, and OFI-EVs from cactus fruits contain plant sphingolipid components that may reduce cryo-recovery damage by stabilizing sperm membrane structure (Valentino et al., 2024), Sesame-derived extracellular vesicles (SAEVs) may also improve fertilization rates by regulating calcium ion channels to promote sperm capacitation (Zhao et al., 2025)

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