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This observed transcriptional shift in the SEC population aligns with established histological reports of sinusoidal rarefaction and loss of integrity in the aged marrow 27,52

Additionally, animal studies such as the one by Gurger et al

Unwanted tissue growth or fibrosis is a legitimate concern when uncoupled to growth peptides are improperly used

Immunoprecipitation Cells were harvested 36 h post-transfection and then lysed in radioimmunoprecipitation assay (RIPA) lysis buffer (50 mM Tris-HCl, 150 mM NaCl, 0.5% sodium deoxycholate, 1% IGEPAL, 1 mM NaF, 1 mM Na3VO4) containing a protease inhibitor cocktail and a phosphatase inhibitor cocktail (Sigma)