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The next day, after a 30-min incubation at RT on a shaker, the sections were washed three times with 1 PBS for 10 min each

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Moreover, H 2 O 2 elicited a rapid NO burst in guard cells of mung bean leaves ( Phaseolus aureus ) (Lum et al., 2002) as well as NOS activity along with PCD in tobacco BY-2 cells (De Pinto et al., 3 ) led to high ROS levels and rapid NO production in the leaves of A