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Samples were diluted with 50 mM Tris-HCl to bring urea concentration down to below 2 M to prevent trypsin inhibition

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Common Side Effects of Tirzepatide and Semaglutide The most frequently reported side effects include: Nausea (particularly during dose increases) Diarrhea or constipation Vomiting Abdominal discomfort Decreased appetite (expected effect) Most side effects occur during dose escalation and typically improve within 4-8 weeks as your body adjusts to the medication

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