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To calculate the cell viability, the cultured cells were uniformly distributed (1 10 3 cells/well) in a cell culture plate and incubated for the next 24 h, and subsequently treated with different concentrations (101000 g/mL) of test and control compounds for the next 5 days similar to cell culture conditions

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In Chinese patients, p.P992L or p.N1270S was associated with presentation before age 12, and p.I1148T occurred almost exclusively in patients presenting after 12 yearsold