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Semi-quantitative analysis of specific extracellular H + level (seH + ) was performed using the ImageJ software by measuring the area of the yellow region (adjust color threshold, make binary, and remove outliers) and the intensity of the yellow color (live histogram) and the root area and by applying the following formula: where A y is the area of the yellow region, I is the color intensity of the yellow region, and A r is the root area

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Third, GSNOs actions likely involve downstream cGMP signaling and inhibition of oxidative stress pathways, as demonstrated in isolated heart models, where GSNO improved post-ischemic recovery via enhanced cGMP formation and coronary flow 47

It is widely accepted that the initial wave of C-fiber activity in phase 1 leads to phase 2, which depends on the inflammatory response and central sensitization