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217 TMM is expected to have better enzymatic performance over the Ruegeria pomeroyi enzyme, so we were sure to include this in our experimentation [7]

For the GSH estimation, 100 L of the supernatant was mixed with 900 L of reaction buffer (0.1 M phosphate buffer, pH 7.5, containing 5 mM EDTA), and the absorbance was measured at 412 nm using a spectrophotometer as the blank value 20

Vitamin B12 deficiency can be caused by several factors, including: Inadequate dietary intake: Since vitamin B12 is naturally found in animal-based foods, strict vegetarians and vegans who do not consume fortified foods or supplements are at risk of developing a deficiency

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