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Cybulski, N., Polak, P., Auwerx, J., Regg, M

Results Disruption of a broad range of metabolites in autochthonous soft-tissue sarcomas In order to better understand the metabolic adaptations that enable the outgrowth of soft-tissue sarcomas (STS), we utilized a genetically engineered mouse model that mimics rhabdomyosarcoma (RMS) that we previously characterized whereby intramuscular injection of 4-hydroxytamoxifen activated a muscle satellite cell-specific CreER T2 to turn on expression of an oncogenic Nras G12D allele and delete both p53 alleles 24 , resulting in local tumor formation (P7NP sarcoma) within 46 weeks (Fig